Method for determining the presence or amount of analyte using a stable colloidal carbon sol
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United States of America Patent
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Jun 25, 1996
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app pub date -
May 12, 1994
filing date -
Nov 19, 1987
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Abstract
This invention relates to a method for determining the presence or amount of an analyte in a sample comprising contacting the sample with a labeled constituent consisting of an aqueous carbon sol which is stable in the absence of a stabilizing substance. The colloidal carbon particles have directly conjugated to their surface a binding component which specifically binds to the analyte and the resulting analyte/carbon particle complex is used as an indication of the presence or amount of analyte in the sample. The grade of carbon used satisfies the condition V>0 wherein V is a linear predictor value according to the formula V=-138.954-0.987.times.DBP+15,609.times.VC+3.994.times.PPD, wherein DBP is the dibutylphthalate adsorption in ml/100 g, as determined according to DIN 53601; VC is the volatile content in %, as determined according to DIN 53552; and PPD is the average primary particle diameter in nanometers.
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