"Alga species lobsphaera TM-33 (ATCC 75630) which is useful for preparing trehalase"
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United States of America Patent
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Jun 25, 1996
Grant Date -
N/A
app pub date -
Apr 22, 1994
filing date -
Dec 28, 1993
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Novel trehalase having the properties that it hydrolyzes .alpha.,.alpha.'-trehalose, 2,2'-dideoxy-.alpha.,.alpha.'-trehalose and 2-deoxy-.alpha.,.alpha.'-trehalose into the respective constituting sugars while it does not act on neotrehalose, lactose, maltose, celobiose and sucrose; its optimum pH is 5 to 6; its optimum temperature is 65.degree. C.; it is stable against heating up to 65.degree. C.; its molecular weight as measured by a gel filtration is 400,000 to 500,000 while the molecular weight of the subunit as measured by a sodium dodecylsulfate--polyacrylamide gel electrophoresis is 180,000 to 250,000; its isoelectric point as measured by an isoelectricfocusing is 2.7; and it is a glycoprotein.
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