Apparatus for automated assay of DNA probe and method for assaying nucleic acid in sample
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United States of America Patent
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Jul 23, 1996
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N/A
app pub date -
Dec 29, 1993
filing date -
Dec 29, 1992
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Abstract
An apparatus is presented for an automated assay of a DNA probe, which is performed by hybridizing a nucleic acid in a sample with a DNA probe marked with an enzyme and optically measuring a change that occurs in the substrate by catalysis of the enzyme which is bound with the DNA probe. The DNA probe hybridizes with the nucleic acid in the sample to assay the nucleic acid in the sample. Comprising (A) a sample-reagent unit, (B) a sample-reagent dispense unit, (C) a reaction vessel transport unit, (D) a hybridization unit, (E) a B/F separation unit, and (F) a light measurement unit; also presented is a method for assaying a nucleic acid in a sample by the apparatus of the present invention. According to the measurement apparatus of the present invention, a continuous and highly precise measurement is attainable. In particular, the light-shielding structure of the light measurement unit exhibits high levels of light-shielding effect and contributes to efficient light measurement and prevention of degradation of light receiving elements.
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