Bacterial mass production of taxanes with Erwinia
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United States of America Patent
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Oct 1, 1996
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app pub date -
May 5, 1995
filing date -
May 5, 1995
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Abstract
The present invention relates to a biologically pure culture of a novel bacteria of genus Erwinia, which is isolated from Taxus canadensis, and wherein said bacteria of genus Erwinia is characterized by the production of paclitaxel and related taxanes in a fermentative or respiratory type of metabolism from simple semi-synthetic or synthetic culture media. Also, the present invention relates to a method of bacterial mass production of paclitaxel and related taxanes which comprises the steps of: a) culturing the bacteria of genus Erwinia of the present invention in a growth-supporting nutrient medium capable of promoting growth and reproduction of said bacteria, and wherein said culturing is effected for a time sufficient to allow production of paclitaxel and related taxanes; and b) isolating said produced paclitaxel and related taxanes from said culturing medium of step a).
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