Methods for coupled high temperatures reverse transcription and polymerase chain reactions
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United States of America Patent
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Oct 1, 1996
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app pub date -
May 24, 1995
filing date -
Aug 22, 1986
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Abstract
Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
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