Quantitative detection of analytes on immunochromatographic strips
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United States of America Patent
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Oct 29, 1996
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N/A
app pub date -
Jan 30, 1995
filing date -
Jan 30, 1995
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Abstract
A method for determining the concentration of analyte in a test fluid by immunochromatography techniques which involves quantitatively determining the signals from captured analyte/labeled binding partner complex by an instrument, e.g. reflectance spectrometer. In a preferred embodiment, a reflectance reading is determined for the captured complex and uncomplexed labeled binding partner which is captured in a separate zone of the immunochromatographic strip and the ratio of these reflectances is used to provide additional quantitation to the assay method.
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