Detection of nucleic acids using a hairpin forming oligonucleotide primer and an energy transfer detection system
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United States of America Patent
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Nov 12, 1996
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N/A
app pub date -
Mar 22, 1993
filing date -
Mar 23, 1992
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Abstract
A process for the detection of a nucleic acid sequence in a homogeneous assay format using an energy transfer system is disclosed. This process utilizes a 5' labeled primer containing a selfcomplementary sequence in an amplification or extension process together with a subsequent detection step using a 3' labeled probe for the amplified or extended region. The labels will be close together in space after hybridizing the probe close to the short piece of double-stranded DNA resulting from backfolding of the selfcomplementary region of the primer which has been incorporated into the amplified or extended product. A new primer for use in this process is also disclosed.
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