Vectors with pectate lyase signal sequence
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United States of America Patent
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Nov 19, 1996
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app pub date -
Dec 9, 1994
filing date -
Nov 1, 1985
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Abstract
The invention relates to DNA coding for a pectate lyase signal peptide which has been inserted into plasmids to create secretion vectors which are capable of producing a chosen protein by directing transport across the bacterial membrane. The secretion vectors have been used to secrete extracellular thaumatin and extracellular antibody fragments. The proteins produced by these vectors have biological activity. The thaumatin is properly folded and the antibody fragments are capable of binding antigens on target cancer cells.
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