There is disclosed a method for determining an endotoxin by reacting the endotoxin adsorbed on an adsorbent having specific endotoxin adsorbability with an endotoxin detecting reagent, including the steps of: (a) preparing a column having a tip opening, a rear end opening and an adsorbent-filling and holding means provided in the column through which a sample solution can pass, (b) introducing the endotoxin adsorbent into the column through the tip opening to fill and hold the adsorbent therein, (c) introducing the sample solution into the column through the tip opening and discharging the introduced sample solution through the rear end opening to contact the sample and the adsorbent and thereby adsorbing the endotoxin on the adsorbent, and (d) reacting the detecting reagent with the endotoxin adsorbed on the adsorbent. There is also disclosed an apparatus for the above method.
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