Method of stimulating proliferation and differentiation of human fetal pancreatic cells ex vivo
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United States of America Patent
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Dec 24, 1996
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app pub date -
Apr 29, 1994
filing date -
Apr 29, 1994
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Abstract
A method of inducing the proliferation and/or differentiation of human fetal pancreatic cells entails contacting such cells in primary culture with Hepatocyte Growth Factor/Scatter Factor, thereby inducing a proliferation of .beta.-epithelial cells, an increase in the number of .beta.-epithelial cells which form islet-like cell clusters, and an increase in insulin production per cell. The method provides increased numbers of functional islet-like cell clusters for transplantation, for example, into Type 1 diabetic patients. The method can be scaled up so as to provide clinically useful numbers of cells for transplantation.
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