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Abstract

A fluorescent label compound having the formula A--B.sup.1 --B.sup.2 --B.sup.3 --C wherein A represents the residue of a natural or synthetic nucleotide or nucleoside or a derivative thereof; B.sup.1 represents a divalent spacer radical or a single bond; B.sup.2 represents a divalent spacer radical; B.sup.3 represents a divalent spacer radical or a single bond; and C represents a coumarin radical having the formula ##STR1## in which R.sup.1 represents hydrogen or cyano; R.sup.2 represents phenyl or thiazolyl bonded in the 2-, 4- or 5-position, wherein the phenyl is unsubstituted or substituted by nitro, cyano, amino, --NH--C.sub.1-4 -alkyl, --(CH.sub.2).sub.1-4 --NH.sub.2, --(CH.sub.2).sub.1-4 --NH--(CH.sub.2).sub.1-3 --CH.sub.3, carboxy, C.sub.1-4 -alkoxycarbonyl, C.sub.1-4 -alkoxycarbonyloxy, hydroxy, C.sub.1-4 -alkylaminocarbonyl, or C.sub.1-4 -alkylcarbonylamino and either further unsubstituted or substituted by C.sub.1-4 -alkyl, fluorine, chlorine or bromine, and where the thiazolyl is unsubstituted or monosubstituted or disubstituted by chlorine, cyano, carboxyl, or C.sub.1-4 -alkoxycarbonyl, or the thiazolyl is fused in the 4- and 5-position with a benzene ring which is either unsubstituted or substituted by carboxyl, amino, or hydroxyl; R.sup.3 represents hydrogen, C.sub.1-4 -alkyl, C.sub.1-4 -alkoxycarbonyl-C.sub.1-4 -alkyl, or phenylsulphonyl, where the C.sub.1-4 -alkyl is unsubstituted or substituted by hydroxyl, amino, carboxyl, or C.sub.1-4 -alkoxycarbonyl, and where the phenylsulphonyl is unsubstituted or monosubstituted or disubstituted by chlorine, bromine, or C.sub.1-4 -alkyl; or where one of the radicals R.sup.2 or R.sup.3 denotes or is substituted by a primary or secondary amino group, hydroxyl, carboxyl, or C.sub.1-4 -alkoxycarbonyl, or can be converted into such a group by hydrolysis or hydrogenation.

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First Claim