Modified N-4 nucleotides for use in amplified nucleic acid hybridization assays
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United States of America Patent
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Jan 14, 1997
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May 10, 1995
filing date -
Oct 15, 1987
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Abstract
Linear or branched oligonucleotide multimers useful as amplifiers in biochemical assays which comprise (1) at least one first single-stranded oligonucleotide unit that is complementary to a single-stranded oligonucleotide sequence of interest, and (2) a multiplicity of second single-stranded oligonucleotide units that are complementary to a single-stranded labeled oligonucleotide. Amplified sandwich nucleic acid hybridizations and immunoassays using the multimers are exemplified.
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