Methods and materials for restriction endonuclease applications
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United States of America Patent
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Feb 18, 1997
Issued Date -
N/A
app pub date -
Dec 22, 1994
filing date -
Mar 24, 1993
priority date (Note) -
Expired
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Abstract
The present invention is directed to materials and methods for the quasi-random and complete fragmentation of DNA using restriction endonuclease reagents capable of cutting DNA at a dinucleotide sequence. The invention is also directed to methods for labeling DNA using template-specific oligonucleotides, for shotgun cloning, for sequencing of DNA, for epitope mapping and for anonymous primer cloning, all using fragments of DNA generated by the method of the present invention.
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