Inhibition of interfering endogenous enzyme activity in assays of biological fluids
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United States of America Patent
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Mar 11, 1997
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app pub date -
Jul 16, 1993
filing date -
Jan 31, 1992
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Abstract
The invention describes biological assays in which hydrogen peroxide is used as an oxidizing agent, or wherein hydrogen peroxide is used to oxidize a dye or other intermediate to generate a detectable species. The stability of the hydrogen peroxide in the presence of at least one other enzyme which decomposes hydrogen peroxide is enhanced by the addition of a suitable inhibitor for the enzyme and the inhibitor does not substantially inhibit enzymes used in the assay. When catalase is the enzyme to be inhibited, catalase inhibitors that can be used in the biological systems include hydroxylamine sulfate. The enzyme inhibitor can be incorporated in an integral analytic device.
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