Methods and compositions for high protein production from recombinant DNA
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United States of America Patent
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Mar 25, 1997
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app pub date -
Jun 5, 1995
filing date -
Oct 25, 1993
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Abstract
Disclosed herein are improved methods and compositions for achieving enhanced protein production expressed from non-native gene constructs, including single chain sFv and derivative sequences. The methods and compositions are particularly useful for creating stably transfected, contitutively expressing immortalized mammalian cell lines that exhibit high recombinant protein productivity while maintaining a low copy number per cell of the non-native recombinant DNA sequence encoding the protein of interest.
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