Fluorescent lipid polymer-macromolecular ligand compositions
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United States of America Patent
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Apr 8, 1997
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N/A
app pub date -
Mar 16, 1995
filing date -
Jul 9, 1993
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Abstract
Methods and compositions are provided for the detection of analytes. The method employs a fluorescence production layer which comprises a fluorescent polymerized polyunsaturated lipid layer in association with a ligand which is a member of a specific binding pair, where the ligand is competitive with the analyte for the complementary binding member or is a complementary binding member. By providing for a fluorescence modulation reagent which binds to the fluorescence production layer in proportion to the amount of analyte in the sample, by measuring the resulting fluorescence after carrying out the assay methodology, the amount of analyte can be determined quantitatively.
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