DNA sequencing by mass spectrometry via exonuclease degradation
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United States of America Patent
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Apr 22, 1997
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Feb 10, 1995
filing date -
Mar 19, 1993
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Abstract
Methods for determining the sequence of nucleic acids by cleaving the nueleic acid unilaterally from a first end with an exonuclease activity to sequentially release individual nucleotides, identifying each of the sequentially release nucleotides by mass spectrometry, and determining the sequence of the nueleic acid from the identified nucleotides are disclosed. The method is amenable to multiplexing for simultaneously determining more than one nucleic acid sequence.
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