Ligation with hammerhead ribozymes

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United States of America Patent

PATENT NO 5633133
SERIAL NO

08275156

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Abstract

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The claimed invention is drawn to a method for ligation of an enzymatic nucleic acid molecule having a hammerhead motif to a separate nucleic acid molecule. The method includes use of ligation enhancing conditions in which ligation is enhanced at least two fold over that observed in a medium containing only Mg.sup.2+ as a divalent cation at 20.degree. C.

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Patent OwnerAddress
LONG DAVID MNot Provided

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hertel, Klemens J 70 Canal St., Medford, MA 02155 1 46
Long, David M Department of Chemistry & Biochemistry, University of Colorado, Campus Box, Boulder, CO 80309-0215 17 470
Uhlenbeck, Olke C Department of Chemistry & Biochemistry, University of Colorado, Campus Box, Boulder, CO 80309-0215 1 46

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