Solution phase nucleic acid sandwich assays having reduced background noise
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United States of America Patent
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Jun 3, 1997
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app pub date -
Apr 26, 1995
filing date -
Dec 8, 1993
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Abstract
New techniques are provided for substantially reducing background signals encountered in solution phase hybridization assays. The techniques are premised on eliminating or significantly reducing the phenomena of nonspecific hybridization and nonspecific binding, so as to provide a detectable signal which is produced only in the presence of the target polynucleotide of interest. In certain embodiments, methods are provided for increasing the signal which can otherwise be diminished in noise reduction. Kits for carrying out the novel assays are provided as well.
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