Importance

Loading Importance Indicators...

Abstract

Compositions for the gel electrophoresis separation of conformational isomers of nucleic acids and agarose compositions useful in such compositions. The compositions use compositions comprising: at least one aqueous gel which is at least one highly derivatized agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 17.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high gel-strength polysaccharide aqueous gel other than polyacrylamide in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount. The invention compositions comprise at least one aqueous gel comprising at least one agarose derivatized sufficiently to reduce its gelling temperature (Tg) to below 9.degree. C., present in 40 wt % or more based upon the gel total weight, in admixture with at least one high strength non-acrylamide polysaccharide present in a balance to 100% total gel weight; and an electrophoretic buffer, present in an electrophoretic buffer--effective amount.

Loading the Abstract Image...

First Claim