Fluorescence polarization detection of nucleic acids
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United States of America Patent
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Jun 24, 1997
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N/A
app pub date -
Nov 15, 1995
filing date -
Nov 15, 1995
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Expired
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Abstract
The present invention provides methods for detecting amplified or unamplified nucleic acid target sequences at increased temperatures by changes in fluorescence polarization. The decrease in fluorescence polarization associated with hybridization of oligonucleotides at higher, more stringent, temperatures is overcome by including a double-stranded DNA binding protein in the assay. At elevated temperatures, the double-stranded DNA binding protein restores, and often enhances, the magnitude of the change in fluorescence polarization associated with single- to double-stranded conversion of an oligonucleotide probe or primer.
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