Use of restriction endonuclease sequences for cleaving phosphorothioate oligonucleotides
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United States of America Patent
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Jul 29, 1997
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N/A
app pub date -
Jun 7, 1995
filing date -
Jun 7, 1995
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Abstract
The present invention relates to methods of cleaving phosphorothioate oligonucleotides. In particular, it relates to the use of certain restriction endonucleases to cleave phosphorothioate oligonucleotides which contain restriction endonuclease recognition sequences. These restriction sequences can be used to facilitate the cleavage of relatively cleavage resistant phosphorothioate oligonucleotides thus facilitating their separation and purification after synthesis.
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