System for automated performance of the polymerase chain reaction

Number of patents in Portfolio can not be more than 2000

United States of America Patent

PATENT NO 5656493
SERIAL NO

08199505

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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There is disclosed herein a machine for performing nucleic acid amplification under computer control. The machine utilizes any one of a number of heating and cooling systems under control of a host computer which directs the heating and cooling systems to heat and cool a reaction-chamber-containing heat exchanger at appropriate times in the process. The reaction chambers are pre-loaded with the nucleic acid(s) to be amplified, a thermostable enzyme to catalyze polymerization, specific oligonucleotide primers, and four different nucleotide triphosphates. Also disclosed is the process for the amplification chain reaction implemented by the machine, which utilizes a thermostable enzyme.

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Patent Owner(s)

Patent OwnerAddressTotal Patents
APPLIED BIOSYSTEMS, LLCFOSTER CITY, CA984

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Johnson, Larry San Jose, CA 47 1293
Leath, Richard A Berkley, CA 3 604
Mezei, Louis M Madison, WI 10 752
Mullis, Kary B LaJolla, CA 32 11644
Wennberg, Timothy J Mariposa, CA 4 634
Widunas, Joseph T Freemont, CA 8 1289

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8409807 NMR systems and methods for the rapid detection of analytes 12 2012
9360457 NMR systems and methods for the rapid detection of analytes 1 2012
8883423 NMR systems and methods for the rapid detection of analytes 3 2012
9562271 species 0 2013
9046493 NMR systems and methods for the rapid detection of analytes 6 2013
9488648 NMR systems and methods for the rapid detection of analytes 1 2013
9702852 NMR systems and methods for the rapid detection of analytes 0 2013
 
THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY (3)
7604983 Apparatus and methods for parallel processing of micro-volume liquid reactions 15 2005
8906618 Apparatus and methods for parallel processing of micro-volume liquid reactions 1 2011
9518299 Apparatus and methods for parallel processing of micro-volume liquid reactions 0 2014
 
DRESSER, INC. (2)
* 2010/0312,401 Chemical Injection System 1 2010
9709995 Chemical injection system 0 2014
 
DAKOCYTOMATION DENMARK A/S (1)
7758809 Method and system for pretreatment of tissue slides 2 2009
 
TRANSNETYX, INC. (11)
7011943 Method for detecting a designated genetic sequence in murine genomic DNA 2 2001
* 2003/0087,286 Isolation of eukaryotic genomic DNA using magnetically responsive solid functionalized particles 4 2002
* 2003/0082,605 Genomic DNA detection method and system thereof 8 2002
7282361 Systems and methods for transgenic and targeted mutagenesis screening 25 2005
* 2005/0170,423 Systems and methods for transgenic and targeted mutagenesis screening 0 2005
* 2005/0221,370 Systems and methods for ordering, performing, and reporting genetic screening 6 2005
* 2006/0014,186 Methods for genotype screening of a strain disposed on an adsorbent carrier 4 2005
* 2005/0272,085 Methods for forensic and congenic screening 2 2005
7494817 Methods for genotype screening using magnetic particles 0 2005
* 2006/0014,192 Methods for genotype screening using magnetic particles 3 2005
* 2005/0266,494 System and method for computer network ordering of biological testing 13 2005
 
WAFERGEN, INC. (1)
* 2006/0030,037 Thermo-controllable high-density chips for multiplex analyses 11 2005
 
DAKO DENMARK A/S (19)
7648678 Method and system for pretreatment of tissue slides 12 2003
8257968 Method and apparatus for automatic staining of tissue samples 7 2003
8216512 Apparatus for automated processing biological samples 1 2003
7960178 Enhanced scheduling sample processing system and methods of biological slide processing 10 2003
7400983 Information notification sample processing system and methods of biological slide processing 58 2003
* 2006/0088,928 Method and apparatus for automatic staining of tissue samples 55 2003
9778273 Isolated communication sample processing system and methods of biological slide processing 0 2003
8298815 Systems and methods of sample processing and temperature control 6 2003
7937228 Information notification sample processing system and methods of biological slide processing 10 2008
8386195 Information notification sample processing system and methods of biological slide processing 8 2011
8394635 Enhanced scheduling sample processing system and methods of biological slide processing 10 2011
8529836 Apparatus for automated processing biological samples 4 2012
8663978 Method and apparatus for automatic staining of tissue samples 3 2012
8788217 Information notification sample processing system and methods of biological slide processing 2 2013
8673642 Enhanced scheduling sample processing system and methods of biological slide processing 4 2013
8784735 Apparatus for automated processing biological samples 0 2013
9599630 Method and apparatus for automatic staining of tissue samples 0 2014
8969086 Enhanced scheduling sample processing system and methods of biological slide processing 1 2014
9229016 Information notification sample processing system and methods of biological slide processing 0 2014
 
SPARTAN BIOSCIENCE INC. (3)
* 2008/0275,229 Method for increasing the speed of nucleic acid amplification reactions 7 2007
8945880 Thermal cycling by positioning relative to fixed-temperature heat source 5 2009
* 2010/0075,296 Thermal cycling by positioning relative to fixed-temperature heat source 1 2009
 
LGC LIMITED (4)
* 7348141 Hybridization beacon and method of rapid sequence detection and discrimination 4 2001
* 2004/0091,864 Hybridisation beacon and method of rapid sequence detection and discrimination 5 2003
7998673 Hybridisation beacon and method of rapid sequence detection and discrimination 1 2008
* 2008/0311,579 Hybridisation beacon and method of rapid sequence detection and discrimination 4 2008
 
STRECK, INC. (5)
9034635 Thermocycler and sample vessel for rapid amplification of DNA 1 2009
* 2011/0039,305 THERMOCYCLER AND SAMPLE VESSEL FOR RAPID AMPLIFICATION OF DNA 0 2009
* 2010/0291,536 SAMPLE PROCESSING CASSETTE, SYSTEM, AND METHOD 16 2010
* 2010/0288,059 SPECIMEN CONTAINER, SYSTEM, AND METHOD 4 2010
9737891 Rapid thermocycler system for rapid amplification of nucleic acids and related methods 0 2012
 
QUALIGEN, INC. (8)
8765367 Methods and instruments for processing a sample in a multi-chambered receptacle 0 2008
8735055 Methods of concentrating an analyte 3 2008
8048375 Gravity-assisted mixing methods 17 2008
7780336 Instruments and methods for mixing the contents of a detection chamber 12 2008
7767447 Instruments and methods for exposing a receptacle to multiple thermal zones 28 2008
8052929 Gravity-assisted mixing methods 24 2011
8480976 Instruments and methods for mixing the contents of a detection chamber 21 2011
8491178 Instruments and methods for mixing the contents of a detection chamber 20 2012
 
ATLAS GENETICS LIMITED (2)
9127308 Nucleic acid probes, their synthesis and use 0 2003
* 2005/0221,315 Nucleic acid probes, their synthesis and use 3 2005
 
ABACUS DIAGNOSTICA OY (2)
7754473 Temperature control of reaction vessel, system with reaction vessel, software product for system and use of system 1 2005
* 2008/0268,434 Temperature Control of Reaction Vessel, System with Reaction Vessel, Software Product for System and Use of System 4 2005
 
TAKARA BIO USA, INC. (7)
7833709 Thermo-controllable chips for multiplex analyses 9 2005
* 2006/0030,035 Thermo-controllable chips for multiplex analyses 5 2005
8252581 Apparatus for high throughput chemical reactions 8 2008
* 2008/0176,290 Apparatus for high throughput chemical reactions 16 2008
9228933 Apparatus and method for multiplex analysis 0 2009
* 2010/0233,698 APPARATUS AND METHOD FOR MULTIPLEX ANALYSIS 2 2009
9132427 Apparatus for high throughput chemical reactions 0 2012
 
SALK INSTITUTE FOR BIOLOGICAL STUDIES (1)
* 2005/0095,627 Multiple antigen detection assays and reagents 19 2004
 
AGILENT TECHNOLOGIES, INC. (6)
6635226 Microanalytical device and use thereof for conducting chemical processes 38 2000
6613560 PCR microreactor for amplifying DNA using microquantities of sample fluid 70 2000
6859050 High frequency contactless heating with temperature and/or conductivity monitoring 11 2002
6730883 Flexible heating cover assembly for thermal cycling of samples of biological material 55 2002
6878905 Apparatus and method for flexible heating cover assembly for thermal cycling of samples of biological material 9 2004
* 2005/0184,042 Method and apparatus for cover assembly for thermal cycling of samples 5 2005
 
National Water Research Institute (1)
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PRESIDENT AND FELLOWS OF HARVARD COLLEGE (1)
9068699 Manipulation of fluids, fluid components and reactions in microfluidic systems 0 2013
 
RUTGERS, THE STATE UNIVERSITY OF NEW JERSEY (2)
7985575 Single protein production in living cells facilitated by a messenger RNA interferase 3 2007
* 2010/0035,346 SINGLE PROTEIN PRODUCTION IN LIVING CELLS FACILITATED BY A MESSENGER RNA INTERFERASE 2 2007
* Cited By Examiner