Process to obtain monoclonal and polyclonal antibodies to identify pathogenic amebiasis and pathogenic Entamoeba histolytica trophozoites
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United States of America Patent
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Aug 26, 1997
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app pub date -
Aug 15, 1990
filing date -
Aug 15, 1990
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Abstract
The present invention is a process to obtain polyclonal and monoclonal antibodies useful to identify symptomatic or asymptomatic amebiasis. This invention includes the following steps: 1) Antigen preparation from electron dense granules (EDGs) secreted by Entamoeba histolytica trophozoites incubated in medium containing collagen type I; 2) EDG purification by differential centrifugation and 3) Production of polyclonal and monoclonal antibodies. Anti-EDG polyclonal antibodies were prepared from the serum of a goat hyperimmunized with EDGs. Monoclonal antibodies were obtained by fusion of spleen cells from Balb/c mice injected with EDG protein and SP2/O-Ag14 cells.
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