US Patent No: 5,665,582

Number of patents in Portfolio can not be more than 2000

Isolation of biological materials

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ATTORNEY / AGENT: (SPONSORED)
 

Importance

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Abstract

A method for the isolation and sorting of biological materials has been developed. Biological material includes chromosomes, segments of chromosomes, cell organelles, or other minute cellular components. The biological material is separated from the cellular milieu, if necessary, and anchored to a support. Examples of a support are glass coverslips, glass or polymer beads. The anchoring is by means of a reversible polymer and cross-linking system. The supported biological material may then be labelled with compositions capable of binding to said material, and with magnetic particles. Examples of the binding material include nucleic acid probes and antibodies. An example of the antibodies would be those directed to histones. Other labels, for example, fluorescein-biotin-avidin may be used. The material may be released from the support and sorted by a magnetic force. This method is an alternative to flow cytometry and presents numerous advantages in terms of time, resolution, purity, and preservation of the structure of the biological material during isolation and separation.

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First Claim

Related Publications

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Patent Owner(s)

Patent OwnerAddressTotal Patents
DEKALB GENETICS CORPORATIONDEKALB, IL37
THE JACKSON LABORATORYBAR HARBOR, ME36

International Classification(s)

Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Kausch, Albert P Stonington, CT 29 742
Narayanswami, Sandya Bar Harbor, ME 2 249

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Patent Citation Ranking

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