Simultaneous dual excitation/single emission fluorescent sensing method for PH and pCO.sub.2
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United States of America Patent
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Sep 30, 1997
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N/A
app pub date -
Sep 12, 1995
filing date -
Sep 12, 1995
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Abstract
A simultaneous dual excitation/single emission ratiometric method using fiber optic sensors is provided for detecting or measuring a parameter of interest in a sample, including pH, concentration of gases such as CO.sub.2, and the like. The method involves simultaneously providing modulated optical light corresponding to first and second regions of an indicator species' absorption or excitation spectrum, detecting modulated emission signals from the indicator species, demodulating the emission signals, calculating the apparent quantity of analyte present in the sample from the indicator emission signals and correcting the apparent quantity of analyte present for variations resulting from external factors, by determining the ratio of the demodulated indicator emission signals. An apparatus is provided for performing the method as well.
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