Two-phase optical assay
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United States of America Patent
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Oct 7, 1997
Grant Date -
N/A
app pub date -
Dec 22, 1994
filing date -
Jun 8, 1993
priority date (Note) -
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status (Latency Note)
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Abstract
A method is provided for measuring an analyte in a sample comprising adding substantially transparent particles to a sample in solution or suspension, said particles having an affinity for said analyte; fractionating the particles from the solution or suspension to form a particle-rich fraction and a substantially particle-free fraction; optically reading the particle-rich fraction at a first and a second wavelength; optically reading the substantially particle-free fraction at at least the first wavelength; and correlating the readings through the particle-rich fraction and the substantially particle-free fraction of the sample, with similar measurements in a particle-containing 'blank' to obtain a quantitative determination of the analyte originally present in the sample.
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