Method for making a polypeptide derivative
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United States of America Patent
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Oct 14, 1997
Grant Date -
N/A
app pub date -
Aug 22, 1996
filing date -
Oct 5, 1994
priority date (Note) -
Expired
status (Latency Note)
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Abstract
A method for making a homogeneous succinylated polypeptide is disclosed. The method can be carried out by adding a succinyl-containing compound to a polypeptide solution, followed by raising the pH of the polypeptide solution to a pH of between about pH 10 to pH 12.5. The succinylation reaction between the succinyl-containing compound and free amino groups of the polypeptide is essentially complete upon raising the polypeptide solution pH to the alkaline pH.
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