Process for the isolation and purification of isoflavones
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United States of America Patent
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Oct 21, 1997
Grant Date -
N/A
app pub date -
Feb 24, 1995
filing date -
Feb 24, 1995
priority date (Note) -
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Abstract
The present invention relates to a process for the isolation and purification of isoflavones from a number of different biomass sources. More particularly, the present invention relates to a three-step process whereby a biomass containing isoflavones with a solvent thereby forming an extract that is subsequently fractionated using a reverse phase matrix in combination with a step gradient elution, wherein the resulting fractions eluted from the column contain specific isoflavones that are later crystallized. The purified isoflavone glycosides may then be hydrolyzed to their respective aglycone.
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