Detection of target nucleic acid molecules using synthesis-deficient thermostable DNA polymerase
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United States of America Patent
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Nov 25, 1997
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Jun 6, 1995
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Dec 7, 1992
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Abstract
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences. In this novel detection method, the signal indicative of the presence of the target nucleic acid sequence is amplified through cleavage of detection molecule(s) which comprise hairpin structures.
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