Nucleotide sequences and methods for detection of Serpulina hyodysenteriae
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United States of America Patent
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Dec 16, 1997
Grant Date -
N/A
app pub date -
Jun 1, 1994
filing date -
Jun 1, 1994
priority date (Note) -
Expired
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Abstract
The invention provides a method for detecting the presence of Serpulina hyodysenteriae in a biological sample, an oligonucleotide primer and an S. hyodysenteriae-specific oligonucleotide probe useful in that method, and an article of manufacture that contains the primers and/or probe. Also provided are an about 2.3-kb DNA fragment derived from genomic DNA of S. hyodysenteriae and encoding for an about 56 kDa polypeptide, a recombinant expression vector containing the DNA fragment, the 56 kDa polypeptide and a monoclonal antibody reactive with the peptide, and a method of assaying for antibodies reactive with the 56 kDa peptide.
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