Method for HLA typing
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United States of America Patent
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Dec 30, 1997
Grant Date -
N/A
app pub date -
Apr 8, 1993
filing date -
Jun 27, 1990
priority date (Note) -
Expired
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Abstract
A method for HLA typing by amplification of a sample followed by sequence-specific oligonucleotide hybridization with a labelled oligonucleotide probe provides for both positive and negative controls. Control sequences representing known allelic polymorphisms at the locus in question are subjected to the labelled probe along with the sample. This method reduces errors and improves the chance of obtaining a successful tissue match, as is vital in the case of tissue transplants, particularly bone marrow transplants. Probes and PCR primers useful in HLA-DR typing are also provided.
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