Nicking of DNA using boronated nucleotides
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United States of America Patent
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Dec 30, 1997
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app pub date -
Aug 22, 1996
filing date -
Aug 22, 1996
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Abstract
.alpha.-Boronated deoxynucleoside triphosphates, when incorporated into a double-stranded restriction endonuclease recognition/cleavage site for a restriction endonuclease, induce nicking by the restriction endonuclease. .alpha.-Boronated deoxynucleoside triphosphates (dNTP.alpha.BH.sub.3) are therefore useful as nucleotide analogs in SDA to produce the nickable hemimodified restriction endonuclease recognition/cleavage site required to sustain the amplification reaction.
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