Buffered embryo solutions
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United States of America Patent
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-
Feb 10, 1998
Grant Date -
N/A
app pub date -
Oct 5, 1995
filing date -
Oct 7, 1994
priority date (Note) -
In Force
status (Latency Note)
Importance
Abstract
Aqueous buffered physiological solutions suitable for use with embryos are disclosed based on a selection of zwitterionic buffers, allowing improved embryo solutions to be produced, giving a greater holding time than conventional phosphate buffers. An embryo flushing solution and an embryo holding solution based on these bufers are described. A preferred buffered embryo holding solution contains the following ingredients: NACl, MOPS (3-?N-morpholino! propane sulfonic acid!), KCl, CaCl.sub.2.2H.sub.2 O, MgCl.sub.2.6H.sub.2 O, NaHCO.sub.3, Kanamycin Sulphate, Glucose, Bovine Albumin, Na Lactate, NaOH, and tissue culture grade water.
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