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Abstract

A method for the immunological determination of proteins or polypeptides which are suitable as tumour tracers, in particular human thyroglobulin, in a sample of a biological fluid. The method comprises placing a sample of the fluid to be tested in solution with immunological binding partners for the protein or polypeptide to be determined and obtaining a signal representative of the amount of the protein or polypeptide, wherein a fixed known amount of the protein or polypeptide to be measured is added to each test sample and to the standards for the calibration curve before the measurement is made. If the signal obtained for the unknown sample is stronger than the signal obtained for the zero standard, the result is considered a positive measured value which is indicative of the presence and amount of the protein or polypeptide in the sample, and if it is weaker the result is considered an indication of a systematic bias of the measured value.

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