Surface modification of polymers with self-assembled monolayers that promote adhesion, outgrowth and differentiation of biological cells
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United States of America Patent
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Feb 24, 1998
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N/A
app pub date -
Apr 28, 1994
filing date -
Mar 6, 1987
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Expired
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Abstract
A process for forming spatially oriented neo-vascular capillaries comprising: (a) providing a combination ultra-thin film (UTF) pattern of at least one cell adhesion promoter and at least one cell adhesion inhibitor wherein the cell adhesion promoters have a linewidth of between about 50-490 .mu.m; (b) seeding the combination UTF pattern with endothelial cells at an initial seeding cell density; (c) adding a first medium for incubating the seeded endothelial cells until the endothelial cells are grown to confluency; (d) replacing the first medium with a second medium at confluency; and (e) allowing the endothelial cells to differentiate into spatially oriented neo-vascular capillaries.
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