CAC H-phosphonate and its use in the synthesis of oligonucleotides
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United States of America Patent
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Mar 10, 1998
Grant Date -
N/A
app pub date -
Mar 31, 1995
filing date -
Apr 24, 1992
priority date (Note) -
Expired
status (Latency Note)
Importance
Abstract
Disclosed herein are N.sup.4 protected deoxycytidines for use in the synthesis of oligonucleotides, the protecting groups being represented by the formula: --CO--(CH.sub.2).sub.0-9 --CH.sub.3. Preferred embodiments are N.sup.4 acetyl deoxycytidines and include N.sup.4 acetyl deoxycytidine phosphoramidites and N.sup.4 acetyl deoxycytidine H-phosphonates. When used to prepare oligonucleotides the protected deoxycytidine compounds provide high quality oligonucleotide products with little side product from cleavage and deprotection reactions carried out with alkyl amine compounds.
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