Method and reagent system for isolation, identification and/or analysis of leukocytes from whole blood samples
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United States of America Patent
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Mar 24, 1998
Grant Date -
N/A
app pub date -
Mar 29, 1996
filing date -
Mar 13, 1987
priority date (Note) -
Expired
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Abstract
A lytic reagent composition and a kit of a lytic reagent system are disclosed for the rapid isolation, identification and/or analysis of at least five subpopulations of leukocytes from a whole blood sample. The composition and system have application to any environment in which the study and/or analysis of the leukocyte fraction of whole blood requires their isolation in their native or near native state. The lytic reagent composition of this invention comprises saponin and a carboxylic acid of the formula RCOOH wherein R is H or a C.sub.1-3 aliphatic hydrocarbon radial optionally substituted by one or more carbonyl and/or hydroxy groups. The kit of a lytic reagent system comprises the lytic reagent composition and a quench reagent.
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