Polynucleotide detection by isothermal amplification using cleavable oligonucleotides
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United States of America Patent
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May 5, 1998
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app pub date -
Sep 29, 1995
filing date -
Sep 29, 1995
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Abstract
A method for detecting a target polynucleotide is provided which relies on the exponential amplification of oligonucleotide fragments. Separated populations of oligonucleotides are provided that contain complementary sequences to one another and that contain at least one scissile linkage which is cleaved whenever a perfectly matched duplex is formed containing the linkage. When a target polynucleotide contacts a first oligonucleotide cleavage occurs and a first fragment is produced which can hybridize with a second oligonucleotide. Upon such hybridization, the second oligonucleotide is cleaved releasing a second fragment that can, in turn, hybridize with a first oligonucleotide in a manner similar to that of the target polynucleotide. Upon such hybridization, cleavage again occurs and an additional first fragment is produced which can hybridize
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