Purification of soluble laminin 5
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United States of America Patent
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Jun 2, 1998
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N/A
app pub date -
Jun 10, 1996
filing date -
Jun 10, 1996
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Expired
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Abstract
A method for purifying soluble laminin 5 from conditioned cell culture medium. A nonionic or anionic detergent is added to conditioned medium to a final concentration of between 0.1% and 1.0%. The conditioned medium is purified by cation exchange chromatography and anion exchange chromatography, yielding laminin 5 of at least about 70% purity.
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