Production and use of normalized DNA libraries
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United States of America Patent
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Jun 9, 1998
Grant Date -
N/A
app pub date -
Jun 18, 1996
filing date -
Jun 18, 1996
priority date (Note) -
Expired
status (Latency Note)
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Abstract
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
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