DNA sequencing, mapping, and diagnostic processes using hybridization and stable isotope labels of DNA
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United States of America Patent
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Jul 14, 1998
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N/A
app pub date -
May 28, 1996
filing date -
May 28, 1996
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Abstract
A DNA sequencing, mapping, and diagnostic process which includes the steps of labeling nucleotide segments or peptide nucleic acids (PNAs) with one or more atoms of specific stable or long-lived radioactive isotopes of a selected element that do not normally occur in DNAs, ODNs or PNAs such that the nucleotide segment or PNA has specific stable or long-lived radioactive isotope of a specified selected element at a terminal or an interior position; hybridizing the labeled nucleotide segment or PNA to complementary nucleic acid segments or PNAs which are fixed on a hybridization surface; and, using mass spectrometric techniques, including RIS, to analyze the presence and position of the labeled hybridized nucleotide segments or PNAs which are bound to the fixed nucleotide segments or PNAs.
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