Apparatus for cryopreparation, dry stabilization and rehydration of biological suspensions
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United States of America Patent
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Jul 14, 1998
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app pub date -
Nov 14, 1996
filing date -
Sep 12, 1990
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Abstract
This invention relates to methods, apparatus and solutions for cryopreserving microscopic biological materials for biologically extended periods of time. The method comprises treating a suspension of biological material, in an appropriate buffer, with a cryoprotectant or combination of cryoprotectants which raises the glass transition temperature range of the sample. One or more dry protectants may be added to the cryosolution. The cryosolution is then nebulized and rapidly cooled with novel apparatus, dried by molecular distillation, stored and then rehydrated in a buffer prior to its use. The solutions comprise novel mixtures of cryoprotectants.
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