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Abstract

Methods and cartridges are provided for the detection of analytes in a sample by using fluid velocity gradients. In the subject methods, the assay medium is prepared by combining sample suspected of containing analyte with a reagent composition which includes at least one of particles and specific binding pair members ('sbpm's '). A circumferential fluid velocity gradient and vertical fluid velocity gradient are applied to the assay medium to affect the formation of agglutinates. The sizes of the resulting agglutinates are related to the velocity gradients. The level of agglutination is detected to determine analyte presence in the sample. In general, a cartridge for employing the assay comprises two spaced apart parallel plates defining an assay area and trough area there between. A sample port and optically clear window are provided. At least one of the plates is connected to a rotational driver to rotate the plate in a direction counter to the position of the other plate.

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First Claim