Process for identifying nucleic acids and triple helices formed thereby
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United States of America Patent
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Aug 4, 1998
Issued Date -
N/A
app pub date -
Nov 12, 1993
filing date -
Oct 30, 1987
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Expired
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Abstract
Probes and processes for their use for specific recognition and/or cleavage of double-stranded DNA or RNA at sequence specific desired loci through the intermediacy of a triple helix are disclosed. These probes may also be used as diagnostic chemotherapeutic agents through incorporation of a radiolabeled, fluorescing, or otherwise detectable molecule. Preferred assay conditions are also provided for recognition of homopurine-homopyrimidine double-helical tracts within large DNA by triple helix formation under physiological conditions. Hybridization probes for double-stranded recognition with binding site sizes that range >8 base pairs are also provided.
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