Method and compositions for high efficiency loading, transfection and fusion of cells by electric pulses
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United States of America Patent
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Aug 4, 1998
Grant Date -
N/A
app pub date -
May 11, 1995
filing date -
May 11, 1995
priority date (Note) -
Expired
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Abstract
Methods and compositions are provided for use in electroloading procedures to increase the transfection and fusion efficiency compared to the methods now used in the art. The compositions comprise a two-phase polymer system containing two water soluble polymers which, when mixed, result in target cells and biological material being encapsulated into one of the polymer phases in a concentrated form. The methods of the present invention for electroloading biological material into target cells comprises mixing the biological material into one of the phases of the two-phase polymer system; mixing the target cells into either of the phases of the two-phase polymer system; mixing the phases together to form an emulsion; and exposing the emulsion to a pulsing electric field in an electroloading process.
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