Reaction mixtures for detection of target nucleic acids
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United States of America Patent
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Sep 8, 1998
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Apr 25, 1995
filing date -
Aug 6, 1990
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Abstract
A process of detecting a target nucleic acid using labeled oligonucleotides uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
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