Oligonucleotides having random riplets
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United States of America Patent
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Sep 15, 1998
Issued Date -
N/A
app pub date -
Jun 5, 1995
filing date -
Aug 24, 1990
priority date (Note) -
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Abstract
The invention provides a method of synthesizing oligonucleotides having random tuplets using individual monomers. The steps consist of: (1) sequentially coupling monomers on separate supports to form at least two different tuplets, the coupling is performed in separate reaction vessels; (2) mixing the supports from the reaction vessels; (3) dividing the mixed supports into two or more separate reaction vessels; and (4) repeating steps (1) through (3) one or more times in the reaction vessels of step (3), wherein the last step ends at step (2). Additionally, the oligonucleotides can be cleaved from the supports.
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