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Abstract

A method is provided for immobilizing a biomolecule coating on the surface of a medical device to obtain improved biocompatibility characteristics for contacting with tissue or body fluids such as blood. The method includes oxidizing a 2-aminoalcohol moiety of a material disposed on the surface with a periodate to form an aldehyde moiety, combining the aldehyde moiety with an amine moiety of a material to bond the aldehyde moiety to the amine moiety through an imine moiety and reacting the imine moiety with a reducing agent to form the coating immobilized on the surface by an amine linkage. Alternatively, the method includes providing on the surface a coating containing the materials having the 2-aminoalcohol moiety and the amine moiety, applying a periodate to oxidize the 2-aminoalcohol moiety to an aldehyde moiety, allowing the aldehyde moiety to react with the amine moiety through an imine moiety and reducing the imine moiety to form an amine linkage crosslinking the 2-aminoalcohol moiety to the amine moiety. The material containing the 2-aminoalcohol moiety or the amine moiety can be a biomolecule or a substrate surface of a medical device. Biomolecules such as proteins or peptides containing a 2-aminoalcohol moiety can be attached to an aminated surface or an aminated biomolecule can be attached to surface having a 2-aminoalcohol moiety.

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