Biologically active fragment of bacillus stearothermophilus DNA polymerase

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United States of America Patent

PATENT NO 5830714
SERIAL NO

08633476

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Abstract

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The present invention is directed to an isolated and purified DNA encoding a biologically active fragment of a thermostable, full length DNA polymerase I enzyme of Bacillus stearothermophilus. More particularly, the invention is directed to a DNA encoding an approximately 66,000 dalton DNA polymerase that lacks 273 amino acids from the N-terminus of the approximately 96,000 dalton B. stearothermophilus DNA polymerase I, and to the protein encoded thereby which has been designated the B. stearothermophilus DNA polymerase I exo- fragment. The enzyme fragments are useful in DNA sequencing, cDNA preparations, thermophilic Strand Displacement Amplification and other molecular biology applications.

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Patent Owner(s)

Patent OwnerAddress
MOLECULAR BIOLOGY RESOURCES INC5520 WEST BURLEIGH STREET MILWAUKEE WI 53210

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Swaminathan, Neela Madison, WI 3 91
Wilkosz, Richard K New Berlin, WI 4 175

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